Skip DNA purification and amplify direct from human whole blood cells or dried blood spots
Utilize Q5 Hot Start High-Fidelity DNA Polymerase for accurate and efficient PCR
Benefit from increased resistance to inhibitors and chemicals with our optimized buffer system
Set up reactions at room temperature with aptamer-based enzyme control
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Product Information
Q5 Blood Direct 2X Master Mix can amplify a wide variety of targets direct from dried blood spots or up to 30% whole human blood, skipping DNA purification. The master mix includes Q5 Hot Start High-Fidelity DNA Polymerase, a thermostable DNA polymerase, with 3′→ 5′ exonuclease activity, fused to a processivity-enhancing Sso7d domain and dNTPs in an optimized buffer with increased resistance to inhibitors in blood, anti-coagulants, and chemicals on filter papers. It is capable of amplifying products up to 7.5 kb from human whole blood cells preserved with sodium EDTA, potassium EDTA, sodium citrate and sodium heparin as well samples stored on common preservative filter papers.
Q5 Hot Start High-Fidelity DNA Polymerase offers robust DNA amplification with low error rates. The addition of an aptamer-based inhibitor allows for room temperature reaction setup.
It is recommended to use NEB’s Tm Calculatorto determine the appropriate annealing temperature for each primer pair and polymerase/buffer of interest. Unlike other calculators, the NEB Tm Calculator takes buffer components that affect melting temperatures into consideration when calculating the best annealing temperature. Other online calculators may underestimate the best Q5 annealing temperature.
Figure 1: Q5 Blood Direct 2X Master Mix enables robust amplification direct from whole or dried blood
Figure 2: Q5 Blood Direct 2X Master Mix offers strong performance on the widest variety of targets
Figure 3: Consistent performance across filter types, blood preservatives and reaction volumes
This product is related to the following categories:
Q5® High-Fidelity DNA Polymerases Products,
Master Mixes Products,
This product can be used in the following applications:
Extraction-Free PCR,
Hot Start PCR,
High-Fidelity PCR,
Reagents Supplied
The following reagents are supplied with this product:
What is the difference between Q5®Blood Direct 2X Master Mix and Hemo KlenTaq (NEB #M0332)?
What types of samples/materials are compatible with the Q5®Blood Direct 2X Master Mix?
What concentration of whole blood is recommended for use with Q5®Blood Direct 2X Master Mix?
What blood anticoagulants can be used with Q5®Blood Direct 2X Master Mix?
How do I pre-treat Dried Blood Spot Punches?
What length of product can be made by Q5® Blood Direct 2X Master Mix?
What ends will my PCR products have?
What are Hot Start and WarmStart® polymerases and when would I use them?
What are the properties of this polymerase (fidelity, product ends, max amplicon, modified base incorporation, etc.)?
My results are not as expected. Where can I find troubleshooting help?
When and how should I use the Q5® High GC Enhancer?
Quality, Safety & Legal
Quality Control Assays
Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
Specifications & Change Notifications
The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
M0500S_L_G_v1
Certificate of Analysis
The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
M0500G_v1_10157482
M0500L_v1_10157480
M0500S_v1_10157478
M0500G_v1_10159686
M0500L_v1_10189911
M0500S_v1_10184748
M0500L_v1_10204369
M0500G_v1_10198149
M0500L_v1_10207348
M0500L_v1_10210691
M0500S_v1_10211736
M0500L_v1_10233224
M0500S_v1_10239513
M0500L_v1_10242551
M0500G_v1_10242497
Safety Data Sheets
The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.
Q5®Blood Direct 2X Master Mix
Legal and Disclaimers
Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.
Licenses
This product is covered by one or more patents.
This product is licensed for research and commercial use from Bio-Rad Laboratories, Inc., under U.S. Pat. Nos. 6,627,424, 7,541,170, 7,670,808, 7,666,645, and corresponding patents in other countries. No rights are granted for use of the product for Digital PCR or real-time PCR applications, with the exception of quantification in Next Generation Sequencing workflows
Behind the paper: Examining Sources of Error in PCR by Single-Molecule Sequencing
Other Products You May Be Interested In
Q5® Hot Start High-Fidelity DNA Polymerase
OneTaq®Quick-Load® 2X Master Mix with Standard Buffer
OneTaq® Quick-Load® DNA Polymerase
Nuclease-free Water
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